Biosynthesis and localization in human fibroblasts
نویسندگان
چکیده
Gerhard LORKOWSKI,* Jos E. ZIJDERHAND-BLEEKEMOLEN,t Ervin G. ERDOS,4 Kurt VON FIGURA* and Andrej HASILIK*§ *Institut fur Physiologische Chemie und Pathobiochemie, Westfalische Wilhelms-Universitat, D-4400 Munster, Federal Republic of Germany, tLaboratory of Cell Biology, University of Utrecht, NL 351 1HG Utrecht, The Netherlands, and $Departments of Anesthesiology and Pharmacology, University of Illinois, College of Medicine, Chicago, IL 60612, U.S.A.
منابع مشابه
Nonspecific lipid transfer protein (sterol carrier protein-2) defective in patients with deficient peroxisomes.
The biosynthesis and intracellular localization of nonspecific lipid transfer protein (nsLTP) in control human subjects and in patients with peroxisome-deficient disorders were investigated. The molecular mass of human nsLTP was indistinguishable from that of rat nsLTP (13 kDa) by immunoblot analysis. Intracellular localization was identical with that of catalase, a marker enzyme of peroxisomal...
متن کاملP-130: Piwil2 Reprograms Human Fibroblasts to Germ Cell Lineage
Background The piwi family genes are highly conserved during evolution and play a crucial role in stem cell self-renewal, gametogenesis, and RNA interference in diverse organisms ranging from Arabidopsis to humans. Piwil2, also known as Hili, is one of the four human homologues of piwi. Piwil2 was found in germ cells of adult testis, suggesting that this gene functions in spermatogonial stem ce...
متن کاملIn vitro Co-Culture of Human Skin Keratinocytes and Fibroblasts on a Biocompatible and Biodegradable Scaffold
Background: Extensive full-thickness burns require replacement of both epidermis and dermis. In designing skin replacements, the goal has been to re-create this model and make a product which has both essential components. Methods: In the present study, we developed procedures for establishing confluent, stratified layers of cultured human keratinocytes on the surface of modified collagen-chito...
متن کاملThe Role of Human Adult Peripheral and Umbilical Cord Blood Platelet-Rich Plasma on Proliferation and Migration of Human Skin Fibroblasts
BACKGROUND Wound healing is a complex and dynamic process following damage in tissue structures. Due to extensive skin damage caused by burn injuries, this study determined the role of human adult peripheral and umbilical cord blood platelet-rich plasma on proliferation and migration in human skin fibroblasts. METHODS Platelet-rich plasma (5, 10, 15, 20 and 50% PRP) from human umbilica...
متن کاملEffect of Low–Level Helium-Neon Laser Irradiation on the Release of Interleukin 6 and Basic Fibroblast Growth Factor from Cultured Human Fibroblasts in High Glucose Medium
Purpose: Low level laser therapy is suggested as a new therapeutic method in diabetic wound healing. This survey aimed to evaluate the effects of low level laser on human fibroblasts cultured in high glucose cultures. Materials and Methods: The human skin fibroblasts were cultured under standard condition. The cells were cultured in high glucose culture medium (15mM/L) for a week and two weeks ...
متن کاملHuman Fibroblast Switches to Anaerobic Metabolic Pathway in Response to Serum Starvation: A Mimic of Warburg Effect
Fibroblasts could be considered as connective tissue cells that are morphologically heterogeneous with diverse functions depending on their location and activity. These cells play critical role in health and disease such as cancer and wound by Production of collagen, fibronectin, cytokines and growth factors. Absence of insulin and other growth factors in serum deprivation condition and similar...
متن کامل